Identification of stable heat tolerance QTLs using inter-specific recombinant inbred line population derived from GPF 2 and ILWC 292

Heat stress during reproductive stages has been leading to significant yield losses in chickpea ( Cicer arietinum L.). With an aim of identifying the genomic regions or QTLs responsible for heat tolerance, 187 F 8 recombinant inbred lines (RILs) derived from cross GPF 2 (heat tolerant) × ILWC 292 sensitive) were evaluated under late-sown irrigated (January-May) and timely-sown environments (November-April) at Ludhiana Faridkot Punjab, India 13 tolerance related traits. The pooled ANOVA both locations traits namely days germination (DG), flowering initiation (DFI), 50% (DFF), 100% (DHF), plant height (PH), pods per (NPP), biomass (BIO), grain (YLD), 100-seed weight (HSW), harvest index (HI), membrane permeability (MPI), relative leaf water content (RLWC) pollen viability (PV)) showed a highly difference RILs. phenotyping data coupled with genetic map comprising 1365 ddRAD-Seq based SNP markers used tolerance. Composite interval mapping provided total 28 23 QTLs, respectively locations. Of these, consensus DG, DFI, DFF, DHF, PH, YLD, MPI have identified Four QTL clusters containing multiple on same region Stable can be one major factors providing as early advantage more seed setting due comparatively longer period. Identified genomics-assisted breeding develop stress-tolerant high yielding cultivars.